Deep UV-LED Based Absorbance Detectors for Narrow-Bore HPLC and Capillary Electrophoresis
نویسندگان
چکیده
منابع مشابه
Deep UV-LED Based Absorbance Detectors for Narrow-Bore HPLC and Capillary Electrophoresis.
The most common detection method for the analytical separation techniques of HPLC and capillary electrophoresis (CE) is absorbance measurement in the deep-UV range (below 300 nm) as a large number of organic species absorb in this wavelength region. Conventional UV detectors are based on deuterium discharge lamps coupled to a monochromator for wavelength selection. Light-emitting diodes (LEDs) ...
متن کاملSurfactant bilayer coatings in narrow-bore capillaries in capillary electrophoresis.
The cationic surfactants didodecyldimethylammonium bromide (DDAB) and dioctadecyldimethyl-ammonium bromide (DODAB) have previously been shown to form semi-permanent coatings that effectively prevent adsorption of cationic proteins in fused silica capillaries with inner diameters of 25-75 µm. This paper investigates the impact that narrower capillary diameters (≤25 µm) have on the stability of s...
متن کاملA multireflection cell for enhanced absorbance detection in microchip-based capillary electrophoresis devices.
The design, fabrication and testing of a photolithographically fabricated, glass-based multireflection absorbance cell for microfluidic devices, in particular microchip-based capillary electrophoresis (CE) systems is described. A multireflection cell was fabricated lithographically using a three-mask process to pattern aluminum mirrors above and below a flow channel in a chip, with 30 microm di...
متن کاملEffect of Capillary Rinsing Protocol on the Reproducibility of Separation in Capillary Electrophoresis with Indirect UV Detection
Capillary electrophoresis (CE) with indirect UV detection is an interesting analytical method for the analysis of drugs and pharmaceuticals. Good and reproducible capillary quality is needed to develop robust methods and to facilitate method transfer in CE. It is widely accepted that preconditioning procedures are indispensable in capillary electrophoresis in order to achieve reproducibility of...
متن کاملFingerprinting of Marsdenia tenacissima by capillary electrophoresis compared to HPLC.
Capillary electrophoresis (CE) is employed for the first time in a fingerprint analysis of Marsdenia tenacissima. Because the CE method is a new approach to fingerprinting, it is necessary to compare it to the conventional one: high-performance liquid chromatography (HPLC). In HPLC separation, 15 components are separated in 55 min, while in CE separation, 10 stable components are separated by 2...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: CHIMIA International Journal for Chemistry
سال: 2015
ISSN: 0009-4293
DOI: 10.2533/chimia.2015.806